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| Agent Name | Application | Mechanism | Solubility | Recommended Protocol |
|---|---|---|---|---|
| Ammonium Sulfate | Protein Purification | Salting Out | Soluble in Water | Gradually add to the protein solution at 0-4°C. |
| Trichloroacetic Acid (TCA) | Protein Precipitation | Denaturation | Insoluble in Water | Add to the sample, incubate on ice, centrifuge. |
| Ethanol | Protein Concentration | Hydrophobic Interaction | Soluble in Water | Mix with protein solution, incubate, then centrifuge. |
| Acetone | Precipitation | Protein Denaturation | Insoluble in Water | Add to sample, mix, and centrifuge. |
| Polyethylene Glycol (PEG) | Protein Precipitation | Concentration | Insoluble in Water | Add to protein solution, incubate, centrifuge. |